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crAss VERSION 2.2
-----------------
Please cite:
"Reference-independent comparative metagenomics using cross-assembly: crAss"
Bas E. Dutilh, Robert Schmieder, Jim Nulton, Ben Felts, Peter Salamon, Robert A. Edwards and John L. Mokili
Bioinformatics 28: 3225-3231 (2012).

Pubmed: http://www.ncbi.nlm.nih.gov/pubmed/23074261
crAss website: http://edwards.sdsu.edu/crass/


USAGE
-----
Run crAss as follows:
perl crAss.pl input_directory

The input directory should contain the metagenomic reads (one file per metagenome)
and a cross-assembly ACE file. Reads files can be in FASTA format (extensions:
.fasta .fna or .fa) or FASTQ format (extensions: .fastq or .fq). Note that read
identifiers should be unique across all the files.

For further info please visit the crAss website at http://edwards.sdsu.edu/crass/


DEPENDENCIES
------------
The PERL script calls the following programs:
- BioNJ                      http://www.atgc-montpellier.fr/bionj/
- GNUPlot                    http://www.gnuplot.info/
- GhostScript                http://www.ghostscript.com/
- DrawTree (Phylip package)  http://evolution.genetics.washington.edu/phylip/getme.html
Make sure these programs are available and executable from your command line, in
the same directory as crAss.pl.
The executables are defined at the top of the crAss.pl script.


COPYRIGHT AND LICENSE
---------------------
Copyright (C) 2012 Bas E. Dutilh

This program is free software: you can redistribute it and/or modify it under
the terms of the GNU General Public License as published by the Free Software
Foundation, either version 3 of the License, or (at your option) any later
version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY
WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A
PARTICULAR PURPOSE.  See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with
this program.  If not, see <http://www.gnu.org/licenses/>.
Source: README.txt, updated 2019-05-03