cash-program

CASH (Comprehensive alternative splicing hunting) is visualized and user-friendly software that aims to self-construct AS (alternative splicing) sites and detect differential AS events between samples of RNA-Seq data. CASH consists of two major stages: SpliceCons (Splice site Construction) and SpliceDiff (differential AS detection). By comprehensively reconstructing AS sites from RNA-seq data, SpliceCons increases the recognition of AS events considerably and subsequently, SpliceDiff uses two combined statistical methods to improve the detection of differential AS events.
  Different from our previous ASD program, we developed a new module named SpliceCons implemented into CASH and also refined the scripts to detect differential AS events. We recommend using CASH, which now replaces the original ASD version.
  You can download CASH from sourceforge or from the website below. Manual is also on the website below.