#12 seq longer than longer than gkpShortReadLength bases, truncating

[Won Cheol Yim]

Dear All,

I try to run CA8.1 for my illumina reads. (PE x 181bp)

But I got this error message.

Can you help me to ran CA?

Thank you.

Won

===========
Starting file '/scratch/wyim/iceplant/wgs/ca/pe.frg'.

Processing INNIE ILLUMINA 1.3+ QV encoding reads from:
'/home/wyim/scratch/iceplant/wgs/source/ec_fastq/ec.paired.A.fastq'
and '/home/wyim/scratch/iceplant/wgs/source/ec_fastq/ec.paired.B.fastq'

GKP finished with 768389442 alerts or errors:
441945432 # ILL Error: seq longer than longer than gkpShortReadLength bases, truncating.
326444009 # ILL Alert: invalid QV in read.
1 # LIB Alert: suspicious mean and standard deviation; reset stddev to 0.10 * mean.

ERROR: library IID 1 'pe_long' has 154.07% errors or warnings.