[wgs-assembler-users] Oxford Nanopore/Illumina Sequence error

[Stephen T. <ste...@im...>]

Hi,

I am trying to assemble some Oxford Nanopore reads with Illumina PE Sequences  using PBcR (8.3rc2). 

So I prepare the Illumina sequences like this

fastqToCA -libraryname illumina_paired_end -technology illumina -type sanger -mates Lamp_R1_001.fastq,Lamp_R2_001.fastq -insertsize 200 50 > illumina_paired_end.frg

and then do the assembly thus:

PBcR -length 500 -partitions 200 -libraryname lamp60 -s oxford.spec -fastq exp16.fastq -genomeSize 60000 illumina_paired_end.frg 

But I get

Error: The PacBio library 0 must be the last library loaded but it preceedes 1. Please double-check your input files and try again. at /package/wgs/8.3rc2/Linux-amd64/bin/PBcR line 1470.

I notice this error had been posted before but I think mine is different  since ' illumina_paired_end' is the illumina library name and ' lamp60' is the assembled library name so there shouldn't be a conflict.

http://sourceforge.net/p/wgs-assembler/mailman/wgs-assembler-users/?viewmonth=201307

Please can some give me some idea what I should do?

Kind regards and thanks,

Steve