[wgs-assembler-users] failure to build genome ovlStore

[Price, S. <s....@uc...>]

Hi all,

Wondered if anyone could help.  Running assembly using illumina and 454 reads, which seemed to be going well until it got to the constructing unitigs stage:

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more 4-unitigger/unitigger.err
/share/apps/genomics/MaSuRCA-2.2.1/CA/Linux-amd64/bin/buildUnitigs: AS_configure()-- AS_CGW_ERROR_RATE set to 0.25

Bubble popping        = on
Intersection breaking = on
Bad mate threshold    = -7
Error threshold       = 0.015 (1.500%)
Error limit           = 0.000 errors
Genome Size           = 0

sizeof(DoveTailNode)  = 32

AS_OVS_openBinaryOverlapFile()-- Failed to open '/home/ucbtpri/mascura2/CA/genome.ovlStore/0001' for reading: No such file or directory
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4-unitigger/ is empty besides the unitigger.err
genome.ovlStore/ is pretty bare - just empty 'idx' and 'ovs'
No longer have any of the .ovb.gz either.

Haven't found any error messages earlier in the process which have helped me to understand what's gone wrong.  Can anyone shed any light on it?

Any help appreciated.  Cheers,
Stephen