[wgs-assembler-users] Number of paired reads per scaffold

[Waldbieser, G. <Geo...@AR...>]

Hi,
In an assembly in which unitigs are produced from a relatively low (4-7X) coverage of longer reads (Sanger, PacBio, Moleculo, hopefully others to come), is a single pair of reads (PE or MP) sufficient to scaffold two contigs, assuming the pair is unique in the genome? Would there be any difference between scaffolding with a unique single pair vs unique multiple pairs that span a particular gap?

Geoff
________________________________
Geoffrey C. Waldbieser
Research Molecular Biologist
USDA, ARS, Warmwater Aquaculture Research Unit
141 Experiment Station Road
Stoneville, MS 38776




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