From: <mic...@ip...> - 2013-11-25 10:10:34
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Hello, After several restarts of an assembly with error-corrected PacBio reads using CA 8.0, i got stuck at the consensusAfterScaffolder stage. Removing consensus.sh and rerunning the assembly couldnt overcome the following error (at end of post). I found a earlier post about consensusAfterScaffolder - problems (in CA 6.0 i think) in the tracker but wasnt sure if its solution is still valid for CA 8.0. Attached is one of the 3 error-files which didnt succeed (from 8-consensus/ ). When looking at the error-files in 8-consensus/ i found two of them terminating with: #IncBaseCount i out of range (possibly non ACGTN letter?)ctgcns: MultiAlignment_CNS.C:193: int IncBaseCount(BaseCount*, char): Assertion `0' failed. # #Failed with 'Aborted' and one with: # Failed with 'Segmentation fault' $less runCA.sge.out.10 ENV: SGE_TASK_ID needs to be unset, done. /data/users/mmoser/Celera/main/assembly20.11.13/8-consensus/peaxi_026 failed -- no .success. /data/users/mmoser/Celera/main/assembly20.11.13/8-consensus/peaxi_056 failed -- no .success. /data/users/mmoser/Celera/main/assembly20.11.13/8-consensus/peaxi_057 failed -- no .success. ================================================================================ runCA failed. ---------------------------------------- Stack trace: at /home/mmoser/CELERA/wgs-8.0/Linux-amd64/bin/runCA line 1501 main::caFailure('3 consensusAfterScaffolder jobs failed; remove /data/users/mm...', undef) called at /home/mmoser/CELERA/wgs-8.0/Linux-amd64/bin/runCA line 5542 main::postScaffolderConsensus() called at /home/mmoser/CELERA/wgs-8.0/Linux-amd64/bin/runCA line 6317 ---------------------------------------- Failure message: 3 consensusAfterScaffolder jobs failed; remove /data/users/mmoser/Celera/main/assembly20.11.13/8-consensus/consensus.sh to try again |