[wgs-assembler-users] Longer reads after correction (pacBioToCA)

[<J....@lu...>]

Hello everyone,

I used pacBioToCA to correct PacBio reads using a unpaired + paired Illumina dataset.
During correction a number of reads were split or trimmed, this is of course expected.
However when I compared the uncorrected reads with the corrected reads I observed that 6.5%
of the reads actually increased in length. When a read is extended it size increases by an average of 32 bp.
One particular read seemed to have been extended > 750 bp!

I was wondering what mechanism actually causes this extension seen in some reads.
Is this normal behavior, have you seen it before, or might there be something off?
I hope someone can enlighten me on this

Thanks (and have a nice weekend),
Jeroen Frank