From: Francois S. <fra...@ir...> - 2013-07-23 08:27:29
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Dear all, We are using Illumina data to correct PacBio runs, as everyone those days... After creating (successufll) the frg file for the illumina, we launched the pacbioToCa system, and it started to run. However after a while, gatekeeper stopped with the following error: "/home/sabotf/sources/wgs/Linux-amd64/bin/gatekeeper -o /data/projects/assembling-glab/tempIlluminaTog5681/asm.gkpStore.BUILDING -F /data/projects/assembling-glab/Illumina_Tog5681clean.frg /data/projects/assembling-glab//tempIlluminaTog5681/IlluminaTog5681.frg > /data/projects/assembling-glab/tempIlluminaTog5681/asm.gkpStore.err 2>&1 ----------------------------------------END Tue Jul 23 09:56:39 2013 (3084 seconds) numFrags = 291073268 Stop requested after 'initialstorebuilding'. ----------------------------------------END Tue Jul 23 09:56:45 2013 (3090 seconds) Error: The PacBio library 0 must be the last library loaded but it preceedes 1. Please double-check your input files and try again. at /home/sabotf/sources/wgs/Linux-amd64/bin/pacBioToCA line 1084." Any idea ? The pacbio input file come from the provider directly, we did not make any change on it Cheers Francois -- -------------------------------------------------------- Francois Sabot, PhD Be realistic. Demand the Impossible. http://bioinfo.mpl.ird.fr/ http://www.mpl.ird.fr/rice ----------------------------------------- UMR DIversity, Adaptation & DEvelopment Centre IRD 911, Av Agropolis BP 64501 34394 Montpellier Cedex 5 France Phone: +33 4 67 41 64 18 ----------------------------------------- |