From: Ole K. T. <o.k...@bi...> - 2012-08-10 19:18:21
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Hi, I ran classify on an Illumina mate pair library, and managed to use one of the old versions of gatekeeper to dump the reads, so I guess they were dumped as innie reads. I thought the library still was outtie, and input that into an assembly. Now, after finishing overlapper (using grid and grid version of overlapStoreBuild) I have a ovlStore of 3.3 TB, so I'd rather not run that again if I can avoid it. I see from this page: http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=Gatekeeper#Library that there are some options in changing orientation of the library, but only "innie" is supported it says. Do you have any suggestions of what I can do? Would it not work changing the library to "outtie"? Thank you. Ole |