I have assembled 100X illumina reads and 1X Pacbio reads with CA7.0 and the genome size is approximately 550Mb.
To assemble illumina reads with 1X Pacbio RS data, I performed correctPacBio with 20X illumina reads.
However, 95% in length (Pacbio reads) were discarded after correctPacBio.
40Mb in length -> 2Mb in length
How to improve the efficiency of correctPacBio?
Shoud I reduce the value of merSize and ovlMerLen?
or Shoud I perform correctPacBio with more illumina reads?
Log in to post a comment.