VFOS

VFOS is a versatile and fast tool for selecting optimal subset of oligonucleotide sequences (barcodes), which uses penalty scores five barcode factors (GC contents, homopolymers, simple sequence repeats with repeat unit of dinucleotides, Hamming distances, and complementarity between barcodes) with appropriate their weights to select optimal barcodes.
Final output barcodes can be used in identification of true variants with very low allele frequency less than or equal to 1% existing in liquid biopsy from cancers, quantitative transcriptome analysis to estimate accurate expression levels of genes or transcripts, and even single-cell sequencing of large cell populations.

Operating system: Linux
Programming language: C++
License: Non-Commercial Research Use Only