Sequencing cDNA using Solexa? If you are flying without a reference genome, aren't fond of the reference genome you have, or are looking for novel transcripts, try looking at our new VCAKE_1.5 tools. VCAKE_1.5 uses the same methods as VCAKE, with additional steps to allow it to assemble exons from Solexa trace data. After your exons are assembled, you can try to link them by way of linkxon.pl, our first step towards creating a tool to assemble exons into full transcripts.... read more