USeq Source Code

AlignmentEndTrimmer: Option -s added to drop reads with -x number of mismatches. If option -e is specified, RNA editing changes are ignored.

Added some new classes, updates to Autoaligner (new sequencing application codes).

Deleted TF straggler

TomatoFarmer Overhaul
1) Works with pysano
2) Code is more organized
3) Can result incomplete jobs
4) Only recalibrates by sample (not by lane)

Updated picard jars. They did a big package rearrangement so had to modify a bunch of classes. Might be a bit painful to get stuff fixed.

Modified RNASeq app to use DESeq2 for the scanning and gene analysis

Relaxed the thresholds for calling splice damaging/ creating variants.

Brought the RNASeq, ChIPSeq, and MultipleReplicaScanSeqs up to speed with DESeq2

Switched the default behavior to merge stranded datasets. These plus minus useq files were causing all kinds of grief.

Cleaned up the code in the VCFComparator. Put in some more catches for NA and INF vals coming back from R