[Useq-users] FPKM measurement for DRDS

[Jesse R. <jes...@u2...>]

I noticed that changing parameters -x and -e in DRDS alters the calculated FPKM. Is this because FPKM is calculated after removal of genes? If so, based on the definition of FPKM, shouldn't this be calculated per million mapped reads and not per million analyzed reads?  


Jesse Rowley
Division of Pulmonary Medicine
University of Utah School of Medicine