Re: [Transdecoder-users] ORFs without ATG as start
Extracting likely coding regions from transcript sequences
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From: Matthew M. <mat...@un...> - 2014-05-14 19:45:29
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Andre, Alternatively, this could be a metric of assembly quality (e.g. http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00013/abstract) which may be a function of the input data. Can you tell us a little bit about the type and quantity of data used in this assembly? Matt __________________________________ *Matthew MacManes*, Ph.D. University of New Hampshire I Assistant Professor Department of Molecular, Cellular, & Biomedical Sciences Durham, NH 03824 Phone: 603-862-4052 I Twitter: @PeroMHC <https://twitter.com/PeroMHC> Web: genomebio.org Office: 189 Rudman Hall I Lab: 145 Rudman Hall On Wed, May 14, 2014 at 2:44 PM, Andre Minoche <And...@cr...> wrote: > > Hi, > > First of all thanks for providing a tool to predict ORFs. > > I am surprised to see, that so many of my ORFs predicted with > transdecoder do not start with ATG (5prime_partial, 650 out of 1807). > > To me it seems like a malfunction of the software. > > Is there an easy way to trim the ORFs to the nearest start codon? > > Thanks > André > > -- > André Minoche, Postdoc > > Centre for Genomic Regulation (CRG) > Doctor Aiguader, 88, 4th floor > 08003 Barcelona > > http://seq.crg.es > > > > ------------------------------------------------------------------------------ > "Accelerate Dev Cycles with Automated Cross-Browser Testing - For FREE > Instantly run your Selenium tests across 300+ browser/OS combos. > Get unparalleled scalability from the best Selenium testing platform > available > Simple to use. Nothing to install. Get started now for free." > http://p.sf.net/sfu/SauceLabs > _______________________________________________ > Transdecoder-users mailing list > Tra...@li... > https://lists.sourceforge.net/lists/listinfo/transdecoder-users > |