Normal Tkinter behaviour (under Win32 Python 2.5) seems to be: if a window has a menu bar at the top, and underlines are set, Alt-[underlined letter] will display the associated submenu (standard behaviour in Win32 apps) without any bindings having to be explicitly created. So if e.g. I do this in Tkinter without Tix...
from Tkinter import *
from Tkconstants import *
root = Tk()
MenuBar = Menu(root)
root.config(menu=MenuBar)
Menu_File = Menu(topMenuBar)
MenuBar.add_cascade(label="File", menu=Menu_File, underline=0)
Menu_File.add_command(label="Testing")
root.mainloop()
...when I run the app holding ALT and pressing the f key will bring up the File menu, even though I've created no bindings. (This is distinct from pressing ALT to shift focus to the menu bar and then pressing f, which seems unaffected by Tix.)
However, when I replace the first line with
from Tix import *
this behaviour stops working. Since this is a deviation from the expected behaviour of native Tkinter widgets, I assume it's a bug introduced by Tix.