Insert Identification requires a user to provide a list of sequences, a tab delimited list of libraries and their barcodes, the sequence of the mutagen used in the experiment and the reference genome to align to. Optionally, metadata describing the libraries can be provided as well.
The steps executed in this phase are as follows:
- Map sequences to libraries and strip barcodes
- Find sequences with the mutagen and strip the mutagen
- Align the sequences to the provided genome
- Identify unique insertions in each library based on the alignments
