Thread: [svtoolkit-help] SV filtering steph (VariantFiltration) not working
Status: Beta
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bhandsaker
From: Thomas Z. <tho...@em...> - 2012-01-20 08:01:58
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Hi, I am running GenomeSTRiP on individual chromosomes which seems to work very well. In most cases, I get the file discovery.vcf as expected. However, in a few cases I only get discovery.unfiltered.vcf (as well as .discovery.unfiltered.vcf.done). When I rerun GenomeSTRiP, the variant filtration is executed again, however, there is still no output, but also no error message. And as far I can see, also the log file doesn't contain any error message. Any idea what the problem might be? Thank you very much! Kind regards Thomas Zichner |
From: Bob H. <han...@br...> - 2012-01-20 15:22:18
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No idea offhand. Have you looked in the log file produced by queue? What I would try is to take the log file from queue, which lists the java command that was run, and run this command directly. If the behavior persists, then maybe truncate the unfiltered.vcf down to just the first record and if the problem still persists, then post the short vcf file. -Bob On 1/20/12 3:01 AM, Thomas Zichner wrote: > Hi, > > I am running GenomeSTRiP on individual chromosomes which seems to work > very well. In most cases, I get the file discovery.vcf as expected. > However, in a few cases I only get discovery.unfiltered.vcf (as well as > .discovery.unfiltered.vcf.done). > When I rerun GenomeSTRiP, the variant filtration is executed again, > however, there is still no output, but also no error message. And as far > I can see, also the log file doesn't contain any error message. > > Any idea what the problem might be? > > Thank you very much! > Kind regards > Thomas Zichner > > ------------------------------------------------------------------------------ > Keep Your Developer Skills Current with LearnDevNow! > The most comprehensive online learning library for Microsoft developers > is just $99.99! Visual Studio, SharePoint, SQL - plus HTML5, CSS3, MVC3, > Metro Style Apps, more. Free future releases when you subscribe now! > http://p.sf.net/sfu/learndevnow-d2d > _______________________________________________ > svtoolkit-help mailing list > svt...@li... > https://lists.sourceforge.net/lists/listinfo/svtoolkit-help |
From: Thomas Z. <tho...@em...> - 2012-01-29 20:42:04
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Dear Bob, thank you very much for your reply! I now ran it on my local machine instead of the cluster and it works. I don't know what the actual problem might have been. The only thing I noticed is that the calling of the filtering step doesn't contain the "-runDirectory" parameter. Could this cause problems? Kind regards Thomas On 20.01.2012 16:21, Bob Handsaker wrote: > No idea offhand. Have you looked in the log file produced by queue? > What I would try is to take the log file from queue, which lists the > java command that was run, and run this command directly. > If the behavior persists, then maybe truncate the unfiltered.vcf down to > just the first record and if the problem still persists, then post the > short vcf file. > -Bob > > On 1/20/12 3:01 AM, Thomas Zichner wrote: >> Hi, >> >> I am running GenomeSTRiP on individual chromosomes which seems to work >> very well. In most cases, I get the file discovery.vcf as expected. >> However, in a few cases I only get discovery.unfiltered.vcf (as well as >> .discovery.unfiltered.vcf.done). >> When I rerun GenomeSTRiP, the variant filtration is executed again, >> however, there is still no output, but also no error message. And as far >> I can see, also the log file doesn't contain any error message. >> >> Any idea what the problem might be? >> >> Thank you very much! >> Kind regards >> Thomas Zichner >> |
From: Bob H. <han...@br...> - 2012-01-29 22:08:03
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That individual step shouldn't require -runDirectory (the input and output files are passed explicitly). It sounds like some transient problem in your cluster environment, but it's hard to tell what it might be. -Bob On 1/29/12 3:41 PM, Thomas Zichner wrote: > Dear Bob, > > thank you very much for your reply! > I now ran it on my local machine instead of the cluster and it works. I > don't know what the actual problem might have been. > > The only thing I noticed is that the calling of the filtering step > doesn't contain the "-runDirectory" parameter. Could this cause problems? > > Kind regards > Thomas > > > > > On 20.01.2012 16:21, Bob Handsaker wrote: >> No idea offhand. Have you looked in the log file produced by queue? >> What I would try is to take the log file from queue, which lists the >> java command that was run, and run this command directly. >> If the behavior persists, then maybe truncate the unfiltered.vcf down to >> just the first record and if the problem still persists, then post the >> short vcf file. >> -Bob >> >> On 1/20/12 3:01 AM, Thomas Zichner wrote: >>> Hi, >>> >>> I am running GenomeSTRiP on individual chromosomes which seems to work >>> very well. In most cases, I get the file discovery.vcf as expected. >>> However, in a few cases I only get discovery.unfiltered.vcf (as well as >>> .discovery.unfiltered.vcf.done). >>> When I rerun GenomeSTRiP, the variant filtration is executed again, >>> however, there is still no output, but also no error message. And as far >>> I can see, also the log file doesn't contain any error message. >>> >>> Any idea what the problem might be? >>> >>> Thank you very much! >>> Kind regards >>> Thomas Zichner >>> > ------------------------------------------------------------------------------ > Try before you buy = See our experts in action! > The most comprehensive online learning library for Microsoft developers > is just $99.99! Visual Studio, SharePoint, SQL - plus HTML5, CSS3, MVC3, > Metro Style Apps, more. Free future releases when you subscribe now! > http://p.sf.net/sfu/learndevnow-dev2 > _______________________________________________ > svtoolkit-help mailing list > svt...@li... > https://lists.sourceforge.net/lists/listinfo/svtoolkit-help |