[svtoolkit-help] Error without description

[Elbay A. <ea...@si...>]

Dear GenomeStrip team,
My name is Elbay Aliyev. I am working as a Research Specialist at Sidra Medical and Research Center. We have huge project of 3000 Qatari Genome Project and we want to use genomestrip in our studies. But we are facing unexplainable error without any stacktrace during Preprocess step without description.
**Preprocess script:
**
java -cp ${classpath} ${mx} \
org.broadinstitute.gatk.queue.QCommandLine \
-S ${SV_DIR}/qscript/SVPreprocess.q \
-S ${SV_DIR}/qscript/SVQScript.q \
-gatk ${SV_DIR}/lib/gatk/GenomeAnalysisTK.jar \
--disableJobReport \
-cp ${classpath} \
-configFile conf/genstrip_installtest_parameters.txt \
-tempDir ${SV_TMPDIR} \
-R data/Homo_sapiens_assembly19.fasta \
-genomeMaskFile data/Homo_sapiens_assembly19.svmask.fasta \
-copyNumberMaskFile data/Homo_sapiens_assembly19.gcmask.fasta \
-genderMapFile data/installtest_gender.map \
-runDirectory ${runDir} \
-md ${runDir}/metadata \
-disableGATKTraversal \
-useMultiStep \
-reduceInsertSizeDistributions false \
-computeGCProfiles true \
-computeReadCounts true \
-jobLogDir ${runDir}/logs \
-I ${inputFile} \
-P chimerism.use.correction:false \
-run \
|| exit 1
and a on a couple of steps we have errors like that.

ERROR 16:09:20,504 FunctionEdge - Error: 'java' '-Xmx2048m' '-XX:+UseParallelOldGC' '-XX:ParallelGCThreads=4' '-XX:GCTimeLimit=50' '-XX:GCHeapFreeLimit=10' '-Djava.io.tmpdir=/gpfs/projects/tmedicine/ealiyev/genomestrip/PMC01/tmpdir' '-cp' '/gpfs/projects/tmedicine/ealiyev/genomestrip/lib/SVToolkit.jar:/gpfs/projects/tmedicine/ealiyev/genomestrip/lib/gatk/GenomeAnalysisTK.jar:/gpfs/projects/tmedicine/ealiyev/genomestrip/lib/gatk/Queue.jar' '-cp' '/gpfs/projects/tmedicine/ealiyev/genomestrip/lib/SVToolkit.jar:/gpfs/projects/tmedicine/ealiyev/genomestrip/lib/gatk/GenomeAnalysisTK.jar:/gpfs/projects/tmedicine/ealiyev/genomestrip/lib/gatk/Queue.jar' 'org.broadinstitute.sv.apps.ComputeDepthProfiles' '-O' '/gpfs/projects/tmedicine/ealiyev/genomestrip/PMC01/PMC01/metadata/profiles_100Kb/profile_seq_GL000232.1_100000.dat.gz' '-I' 'PMC01/metadata/headers.bam' '-configFile' 'conf/genstrip_installtest_parameters.txt' '-P' 'chimerism.use.correction:false' '-R' 'data/Homo_sapiens_assembly19.fasta' '-L' 'GL000232.1:0-0' '-genomeMaskFile' 'data/Homo_sapiens_assembly19.svmask.fasta' '-md' 'PMC01/metadata' '-profileBinSize' '100000' '-maximumReferenceGapLength' '10000'
ERROR 16:09:20,509 FunctionEdge - Contents of /gpfs/projects/tmedicine/ealiyev/genomestrip/PMC01/PMC01/logs/SVPreprocess-111.out:
INFO 16:04:10,442 HelpFormatter - -------------------------------------------------------------
INFO 16:04:10,445 HelpFormatter - Program Name: org.broadinstitute.sv.apps.ComputeDepthProfiles
INFO 16:04:10,449 HelpFormatter - Program Args: -O /gpfs/projects/tmedicine/ealiyev/genomestrip/PMC01/PMC01/metadata/profiles_100Kb/profile_seq_GL000232.1_100000.dat.gz -I PMC01/metadata/headers.bam -configFile conf/genstrip_installtest_parameters.txt -P chimerism.use.correction:false -R data/Homo_sapiens_assembly19.fasta -L GL000232.1:0-0 -genomeMaskFile data/Homo_sapiens_assembly19.svmask.fasta -md PMC01/metadata -profileBinSize 100000 -maximumReferenceGapLength 10000
INFO 16:04:10,453 HelpFormatter - Executing as ea...@hp... on Linux 3.10.0-229.el7.x86_64 amd64; Java HotSpot(TM) 64-Bit Server VM 1.8.0_121-b13.
INFO 16:04:10,454 HelpFormatter - Date/Time: 2017/09/24 16:04:10
INFO 16:04:10,454 HelpFormatter - -------------------------------------------------------------
INFO 16:04:10,454 HelpFormatter - -------------------------------------------------------------
INFO 16:04:10,461 ComputeDepthProfiles - Opening reference sequence ...
INFO 16:04:10,462 ComputeDepthProfiles - Opened reference sequence.
INFO 16:04:10,462 ComputeDepthProfiles - Opening genome mask ...
INFO 16:04:10,463 ComputeDepthProfiles - Opened genome mask.
INFO 16:04:10,465 MetaData - Opening metadata ...
INFO 16:04:10,466 MetaData - Adding metadata location PMC01/metadata ...
INFO 16:04:10,476 MetaData - Opened metadata.
INFO 16:04:10,476 ComputeDepthProfiles - Opened metadata.
INFO 16:04:10,476 ComputeDepthProfiles - Initializing input data set ...
INFO 16:04:10,513 ComputeDepthProfiles - Initialized data set: 1 file, 1 read group, 1 sample.
INFO 16:04:10,518 MetaData - Loading insert size histograms ...
INFO 16:04:11,940 ReadCountCache - Initializing read count cache with 1 file.
INFO 16:04:12,010 CommandLineProgram - Program completed.
Done. There were no warn messages.
Looks like script did his job perfect but still gives error. Same issue with SVPreprocess 6 and 11 [:(]
echo "./discovery.sh" | bsub -n 16 -e /gpfs/projects/tmedicine/ealiyev/genomestrip/PMC01/test.err -o /gpfs/projects/tmedicine/ealiyev/genomestrip/PMC01/test.out -P PMC01.test
We are submitting to our LSF system like that. our LSF version is 10.1. Java version is 1.8.21. GATK we are using internal one provided with GATK.
Thanks in advance.

Elbay Aliyev
Software Engineer

Sidra Medical and Research Center
PO Box 26999 | Doha, Qatar
Direct: 40037393
ea...@si...<mailto:ea...@si...> | www.sidra.org<http://www.sidra.org/>

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