Re: [svtoolkit-help] Error message in SVDiscovery step
Status: Beta
Brought to you by:
bhandsaker
From: Bob H. <han...@br...> - 2011-09-01 15:47:56
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What version? -Bob On 9/1/11 11:44 AM, Kim Wong wrote: > Hi Bob > > I'm running the Discovery phase using 10Mb windows and getting this error: > > INFO 16:04:17,165 SVDiscovery - Processing cluster > 1:207788701-207788845 1:207889639-207889798 LR 6 > [GC 1278669K->267636K(1586432K), 1.2806820 secs] > [GC 1375348K->267488K(1594112K), 1.2806950 secs] > [Full GC 267488K->160084K(1605440K), 0.3875220 secs] > #DBG: RC Cache fill 1:207778846-207888845 110000 100 > 9.229631 sec > Error: Exception processing cluster: null > Cluster: 1:207788701-207788845 1:207889639-207889798 LR 6 > [GC 591509K->161512K(1456512K), 1.2747640 secs] > [Full GC 161512K->39951K(1456512K), 0.1467510 secs] > ##### ERROR > ------------------------------------------------------------------------------------------ > ##### ERROR stack trace > java.lang.IndexOutOfBoundsException > at java.nio.Buffer.checkIndex(Buffer.java:514) > at java.nio.DirectByteBuffer.get(DirectByteBuffer.java:209) > at > org.broadinstitute.sv.discovery.ReadCountCache2.getReadCounts(ReadCountCache2.java:154) > at > org.broadinstitute.sv.discovery.ClusterDepthModule.getReadCounts(ClusterDepthModule.java:215) > at > org.broadinstitute.sv.discovery.ClusterDepthModule.computeDepth(ClusterDepthModule.java:137) > at > org.broadinstitute.sv.discovery.DeletionDiscoveryAlgorithm.processCluster(DeletionDiscoveryAlgorithm.java:415) > at > org.broadinstitute.sv.discovery.DeletionDiscoveryAlgorithm.processClusters(DeletionDiscoveryAlgorithm.java:323) > at > org.broadinstitute.sv.discovery.DeletionDiscoveryAlgorithm.runDiscovery(DeletionDiscoveryAlgorithm.java:173) > at > org.broadinstitute.sv.discovery.SVDiscoveryWalker.onTraversalDone(SVDiscoveryWalker.java:165) > at > org.broadinstitute.sv.discovery.SVDiscoveryWalker.onTraversalDone(SVDiscoveryWalker.java:44) > at > org.broadinstitute.sting.gatk.executive.Accumulator$StandardAccumulator.finishTraversal(Accumulator.java:129) > at > org.broadinstitute.sting.gatk.executive.LinearMicroScheduler.execute(LinearMicroScheduler.java:85) > at > org.broadinstitute.sting.gatk.GenomeAnalysisEngine.execute(GenomeAnalysisEngine.java:236) > at > org.broadinstitute.sting.gatk.CommandLineExecutable.execute(CommandLineExecutable.java:116) > at > org.broadinstitute.sv.main.SVCommandLine.execute(SVCommandLine.java:110) > at > org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:221) > at org.broadinstitute.sv.main.SVCommandLine.main(SVCommandLine.java:72) > at org.broadinstitute.sv.main.SVDiscovery.main(SVDiscovery.java:21) > > > The parameters were set to: > -minimumSize 100 > -maximumSize 1000000 > -windowSize 10000000 > -windowPadding 10000 > > which gives for this window: > > -partitionName P0021 -filePrefix P0021 -L 1:199990000-211010000 > -searchLocus 1:200000000-209999999 -searchWindow 1:199990000-211010000 > -searchMinimumSize 100 -searchMaximumSize 1000000 > > Any idea what is wrong? > > Thanks > > Kim > > > > > > |