New Year, new Problem ;)
I was looking for a function which designs me some walk and quality primer=
s on my assembly.
The "Suggest Primers" functions seems to do what I need.
Reading the manual says:
"The purpose of this function (which is available from the gap4 Experiment=
s menu) is to suggest custom primer experiments to extend and "double stra=
nd" contigs. First the routine finds regions of contigs with data on only =
one strand. Then it selects templates and primers, which if used in sequen=
cing experiments, would produce data to cover these single stranded region=
Sounds good and works really fine for walk primer design but not a single =
quality primer is created.
So I removed selective some readings to be sure to have a single stranded =
area. This region is about 100 bases long but again no quality primer was =
Is there some hidden Parameter I missed or should single stranded area be =
I would be glad if you could point me into the right direction.
With kind regards
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