## staden-package — General purpose discussion related to the package.

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 [Staden-package] "Suggest Primers" function and quality primers From: - 2007-01-05 08:32:54 ```Hello, New Year, new Problem ;) I was looking for a function which designs me some walk and quality primer= s on my assembly. The "Suggest Primers" functions seems to do what I need. Reading the manual says: "The purpose of this function (which is available from the gap4 Experiment= s menu) is to suggest custom primer experiments to extend and "double stra= nd" contigs. First the routine finds regions of contigs with data on only = one strand. Then it selects templates and primers, which if used in sequen= cing experiments, would produce data to cover these single stranded region= s." Sounds good and works really fine for walk primer design but not a single = quality primer is created. So I removed selective some readings to be sure to have a single stranded = area. This region is about 100 bases long but again no quality primer was = created. Is there some hidden Parameter I missed or should single stranded area be = longer=3F I would be glad if you could point me into the right direction. Thank you. With kind regards Stefan Kesberg =5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F=5F Viren-Scan f=FCr Ihren PC! Jetzt f=FCr jeden. Sofort, online und kostenlos. Gleich testen! http://www.pc-sicherheit.web.de/freescan/=3Fmc=3D022222 ```

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