I have sequenced 96 PCR products (16S rRNA genes) from both ends using T3 and T7 sites on the cloning vector. Can anyone point me to a Config File or Naming Scheme file for doing a directed assembly with these paired end reads?
***Also, I have repeated the sequencing, so I also need a Config File or Naming Scheme File for a directed assembly of 2 sets of paired end reads. That is, I have 2 reads from the same T3 sequencing primer and 2 reads from the same T7 sequencing primer and I want to use all 4 reads in the directed assembly.
no answers here yet? where else might i look for help?
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