Im trying to set up a blank database using pregap4 and once I run the programe I get this error in the error window. This only happened with one gene files, the other genes I did were fine.
Create_Tag_ for_gel: Tag has negative length
Create_Tag_ for_gel: Tag overlaps gel reading end – not entered
What does it mean? can I ignore it? how can I correct it?
Thank you for your help
It occurs when a tag has incorrect start/end coordinates, either with start > end or with coordinates taking the tag outside the boundaries of the read it is tagging.
I do not know why this would be so though. It is relatively harmless in that (I think) it simply rejects the tag and continues as before. However it would be good to know what causes the tag to be incorrect. You should see some TG lines in the experiment file associated with that sequence. They are the tag lines and maybe will explain where the offending tag is coming from.
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