Re: [scipion-users] Import particles from cryoSPARC

[Grigory S. <sha...@gm...>]

Hi,

I think you could use the import particles protocol and provide a cs file
directly, without need for any motion correction or ctf estimation in
scipion.

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Mon, Mar 27, 2023 at 2:16 PM Ioannis Skalidis <joh...@gm...>
wrote:

> Dear all,
>
> I started an analysis in Scipion of a dataset I have originally analyzed
> in cryoSPARC. I redid the motion correction and ctf estimation in
> Scipion and ideally, I would like to import the already clean particle
> set I have from the cryoSPARC workspace. Could you advise me on how to
> proceed?
>
> Should I use the "import particles" tool? Is it possible to directly
> import the extracted single-particle images, or do I just import the
> particle locations and then re-extract from the newly, in-scipion motion
> corrected images?
>
> Thank you for your input!
>
> Ioannis.
>
> --
>
>
>
> Skalidis Ioannis, MSc
> Biochemistry & Cryo-EM Scientist
> PhD Candidate
> Kastritis Laboratory for Biomolecular Research
> Cryo-Electron Microscopy & Computational Structural Biology
>
> ________________________________________________
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