[scipion-users] Rotate_Particles function is no longer available?

[Leonardo T. R. <leo...@us...>]

Dear Scipion team,
I hope you are well.

I´m working with a Phage in which I need to change the symmetry axis of the
particles and the 3D volume in order to do a symmetry_relaxation
refinement. I saw similar approaches suggested in the links below.

https://github.com/OPIC-Oxford/localrec/wiki/Tips-and-tricks#TnT-2 -
Reorienting all particles
https://www.nature.com/articles/s41467-021-24455-4 -
Methods section - Decoupling *D* 3d symmetry for asymmetric (*C* 1)
reconstruction

However, the relion_rotate_particles.py is not recognized in the scipion
command line, and I do not see a way to do a similar job using the scipion
GUI. I installed the latest version of LocalRec plugin, but still the
command is not recognised.
Am I missing something obvious or this function has been disabled in the
latest updates for some reason?
Any other suggestions on how to do this rotation would also be welcome.

Thank you for your time,
Kind wishes,
Leonardo

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Leonardo TALACHIA ROSA, PhD
PostDoctoral Researcher
Microbiology and Structural Biology
Instituto de Química, Universidade de São Paulo - IQ-USP
Av. Prof. Lineu Prestes, 748 - Butantã, São Paulo - SP
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