Re: [scipion-users] Error in Scipion when using relion extract particles

[Grigory S. <sha...@gm...>]

Hi Adria,

Could you please create an issue
<https://github.com/scipion-em/scipion-em-relion/issues/new?assignees=&labels=&template=bug_report.md&title=>
for
this?

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Mon, Feb 14, 2022 at 1:26 PM Adria Alcaide Jimenez <aa...@ib...>
wrote:

> Hello Scipion team,
>
> I have a question regarding Relion extract particles.
>
> When I do a first particle picking (xmipp) and I extract the particles I
> have no problem but
> when I get 2D classes with Relion and I perform an autopicking with Relion
> and the 2D reference averages, I can not extract the particles with Relion
> extract particles.
>
> The error that appears is : "local variable 'posSet' referenced before
> assignment".
>
> I have tried to extract particles with Xmipp and I can, but then when I
> perform the 2D classification with Relion 2D class it appears the same
> error. I don't know what does it mean or how can I solve it.
>
> Do you have any idea?
>
> Thank you in advance for your help,
>
> Adrià.
>
> --
> Adrià Alcaide i Jiménez
> *PhD student*
> Structural Biology Unit (SBU)
> Consejo Superior de Investigaciones Científicas (CSIC)
> Instituto de Biología Molecular de Barcelona (IBMB)
> C/Baldiri i Reixac 10-12 Edifici Cluster
> 08028 Barcelona (Spain)
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