Re: [scipion-users] Comparing 2 volume maps

[Estrella F. G. <me....@cn...>]

Hello Dmitry,

I suggest you to use some of the new programs in Xmipp (and of course  
integrated in the correspondant Scipion plugin), which are actually  
implemented for this kind of task.
The programs are:

xmipp3 - volumes adjust: it allows you to have the volumes in the same  
numerical frame, thus, you can compare there in a reliable way (same  
threshold).

xmipp3 - volumes subtraction: it performs the alignment described  
above and then it computes the subtraction between the volumes, thus,  
it result should be the differences between the volumes.

xmipp3 - volume consensus: applying first "volume adjust protocol"  
described above, then this protocol is able to make a fusion of a set  
of volumes, taking the best parts of each one. The result will be a  
volume consensus between the input. The result is automatically  
displayed in chimera with a colormap that indicates you the degree of  
similarity among the input maps (what we call a "difference map").

You can find more details of these protocols in this paper:  
https://www.sciencedirect.com/science/article/pii/S104784772100085X?via%3Dihub

This three protocols have to be applied once the set of volumes has  
the same box size and pixel size (you can use xmipp3 - crop/resize  
protocol to achive this). Also, the volumes have to be properly  
aligned (using xmipp3 - align volume, for example).

Feel free to contact me in case you have any doubt. Hope it helps!

Regards,
Estrella FG


Dmitry Semchonok <Sem...@gm...> escribió:

> <>Dear colleagues,
>
>
>
> My task is to compare 2 volume maps to see the differences. The maps  
> are biologically pretty similar.
>
> What strategy shall I undergo and tool to select?
>
>
>
> Apart from biological similarities they are different for the pixel  
> size, box size, resolution and volume threshold.
>
>
>
> Thank you
>
>
>
> Sincerely,
>
>  <>
> Dmitry