Re: [scipion-users] Per-particle CTF?

[Colin D. <ckd...@gm...>]

I do have gctf 1.06 installed but I don't see any option to do a gradient
ctf estimation in the protocol. Just to clarify I'm planning to do this at
the beginning of data processing, not after I have a map etc. via particle
polishing. (Images below to show what I'm looking at)

[image: image.png]
[image: image.png]

On Tue, Sep 14, 2021 at 12:19 PM Grigory Sharov <sha...@gm...>
wrote:

> Hi Colin,
>
> gctf refinement protocol only works with gctf 1.06, which is not installed
> by default. You can run "scipion installb gctf-1.06" to get it.
>
> Best regards,
> Grigory
>
>
> --------------------------------------------------------------------------------
> Grigory Sharov, Ph.D.
>
> MRC Laboratory of Molecular Biology,
> Francis Crick Avenue,
> Cambridge Biomedical Campus,
> Cambridge CB2 0QH, UK.
> tel. +44 (0) 1223 267228 <+44%201223%20267228>
> e-mail: gs...@mr...
>
>
> On Tue, Sep 14, 2021 at 7:29 PM Deniston, Colin <
> col...@no...> wrote:
>
>> Hi,
>>
>>
>>
>> I want to run per-particle CTF (or generate a gradient of CTF values
>> across an image) on some tilted images. However, I can’t find an option for
>> this is my standard gctf – ctf estimate protocol. Could you help me locate
>> it or let me know what plugin performs it I can download? Thanks!
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