Re: [scipion-users] import volumes with half maps question

[Marta M. <mm...@cn...>]

Hi Wolfgang,

I've tested the import volumes protocol and I've had no problems with  
SCIPION v3.0.9-Eugenius.

I think that you can solve your problem filling in the "Files  
directory" with the path of the main map  
(/Runs/000500_ProtRelionRefine3D/extra/relion_class001.mrc) and then  
the "Path half map1" and "Path half map2" with the respective paths  
(/Runs/000500_ProtRelionRefine3D/extra/relion_half1_class001_unfil.mrc  
and  
/Runs/000500_ProtRelionRefine3D/extra/relion_half2_class001_unfil.mrc). I've  
tested this example and it works. ("Pattern" box is empty).

However, the two half maps that you can import this way are not  
SCIPION objects, just the main map. The half maps are attributes of  
the main map and then they will not be recognized as maps by other  
protocols. So, if you want to use any of those half maps as input for  
a job in SCIPION I recommend you to import each one independently in  
an individual import volumes protocol writing its respective whole  
path in the box "Files directory". Sorry for the inconvenience.

With best wishes: MARTA


Quoting "Lugmayr, Wolfgang" <w.l...@uk...>:

> Hi,
>
> I wanted to import 2 half-maps with the import volumes job.
>
> I selected the 2 maps with full path in the file browser and no  
> files directory or pattern:
> When I press execute it says:
> - The path and pattern can not be both empty!!!
>
> When I set also the files directory - error:
> - Folders can not be selected.
>   /home/lugmayr/foldername/
>
> When I set a file pattern + a files directory - error:
> - You can not use the options 'Pattern' and 'Set half maps' simultaneously
>
> Do I miss something here?
>
> How do I select a half map from a relion refinement job as input for a job?
>
> Cheers,
> Wolfgang
>
>
> --
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-- 
Marta Martinez Gonzalez

Biocomputing Unit (Lab B13)
National Center for Biotechnology-CSIC
Darwin, 3. Campus de la Universidad Autonoma de Madrid
28049 Madrid. Spain
Tel:+34 915854510 Fax:+34 913720112
E-mail: mm...@cn...