Re: [scipion-users] Relion -- motion correction

[Grigory S. <sha...@gm...>]

Please check first if the input movies and aligned mics are okay for black
particles.

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Wed, Jul 14, 2021 at 11:00 AM Dmitry Semchonok <Sem...@gm...>
wrote:

> Dear Grigory,
>
>
>
> You say
>
> I don't see any difference between the two screenshots.
>
> *The difference is the presence of trumbnail of movie + psd on old result
> and absence on new (may be due to the version difference?)*
>
>
>
>
>
> Let me explain the all problem.
>
>
>
> I am currently try to find the reason why I have the error during 3D
> refinement in both relion & cryosparc.
>
>
> Bayesian polishing:
>
> During that step I received the first warning — the outcome images were
> containing the black squares sometimes
>
>
>
>
>
>
>
> Then I tracked to relion - motion correction where I noticed that the
> previous run with relion - motion correction (on the same dataset) have a
> trumbnail of movie + psd
>
> Then I decided to do the CTF estimation (with gctf as previously) on the
> suspicious relion- motion correction result and the outcome was wrong as
> well.
> Previously the output was fine
>
> (The images are identical — the differences may be in the version of
> relion-motion cor!)
>
>
>
>
>
>
> This one are the old results
>
>
>
>
>
>
> So what do you thing may cause the error in the refinement/polishing-?
>
>
> Thank you
>
>
> Sincerely,
> Dmitry
>
>
>
>
>
>
> On 14. Jul 2021, at 11:12, Grigory Sharov <sha...@gm...>
> wrote:
>
> HI don't see any difference between the two screenshots.. The first movie
> is taken on carbon, so shifts are much smaller (look at the axes) compared
> to the second "normal" movie in ice.
>
>
> Best regards,
> Grigory
>
>
> --------------------------------------------------------------------------------
> Grigory Sharov, Ph.D.
>
> MRC Laboratory of Molecular Biology,
> Francis Crick Avenue,
> Cambridge Biomedical Campus,
> Cambridge CB2 0QH, UK.
> tel. +44 (0) 1223 267228 <+44%201223%20267228>
> e-mail: gs...@mr...
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