Re: [scipion-users] Relion -- motion correction

[Dmitry S. <Sem...@gm...>]

Dear Grigory,



You say
> I don't see any difference between the two screenshots.
The difference is the presence of trumbnail of movie + psd on old result and absence on new (may be due to the version difference?)





Let me explain the all problem.



I am currently try to find the reason why I have the error during 3D refinement in both relion & cryosparc.


Bayesian polishing:

During that step I received the first warning — the outcome images were containing the black squares sometimes








Then I tracked to relion - motion correction where I noticed that the previous run with relion - motion correction (on the same dataset) have a trumbnail of movie + psd

Then I decided to do the CTF estimation (with gctf as previously) on the suspicious relion- motion correction result and the outcome was wrong as well.
Previously the output was fine

(The images are identical — the differences may be in the version of relion-motion cor!)


 




This one are the old results







So what do you thing may cause the error in the refinement/polishing-?


Thank you


Sincerely,
Dmitry






> On 14. Jul 2021, at 11:12, Grigory Sharov <sha...@gm...> wrote:
> 
> HI don't see any difference between the two screenshots.. The first movie is taken on carbon, so shifts are much smaller (look at the axes) compared to the second "normal" movie in ice.
> 
> Best regards,
> Grigory
> 
> --------------------------------------------------------------------------------
> Grigory Sharov, Ph.D.
> 
> MRC Laboratory of Molecular Biology,
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