Re: [scipion-users] postprocessing to gain resolution

[Jose L. V. <jlv...@gm...>]

Dear Dmitry,

Sharpening algorithms do not change the FSC-resolution of the map. They
only enhance the visualization. The reason why in relion-postprocessing the
FSC changes is because of masking, but not because of the high frequency
boosting that the postprocessing does.

So summarizing, sharpening approaches do not change the resolution of the
map.

Same occurs for local resolution methods based on SNR (blocres, MonoRes and
ResMap). You can see good practices in local resolution here (
https://www.sciencedirect.com/science/article/abs/pii/S0959440X20301044)


Best

Jose Luis Vilas




El mié, 30 jun 2021 a las 8:47, Dmitry A. Semchonok (<sem...@gm...>)
escribió:

> Dear colleagues,
>
>
>
> What are the strategies to push the resolution higher after 3D refinement?
>
>
>
> In SCIPION the available are:
>
> xmipp – localdeblur sharpening
>
> xmipp – deepEMhancer
>
> relion – post-processing.
>
>
>
> It seems that only relion from those gives the FSC.
>
> Am I correct?
>
>
>
>
>
> Thank you.
>
>
>
> Sincerely,
>
> Dmitry
>
>
> _______________________________________________
> scipion-users mailing list
> sci...@li...
> https://lists.sourceforge.net/lists/listinfo/scipion-users
>