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Re: [scipion-users] How to get a multi-color orientation distribution picture from Cryo-EF
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From: 范宏成 <187...@16...> - 2021-06-21 02:07:39
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Hi Grigory and Pablo, Thanks for your response and help! I will try it Best regards, Hongcheng Fan | | 范宏成 | | m18...@16... | 签名由网易邮箱大师定制 On 6/21/2021 04:49，<pc...@cn...> wrote： You're great! This is called fast response time! I actually didn't know there was a cryoEF plugin. 🤦♂️ El 20 jun. 2021 15:22, Grigory Sharov <sha...@gm...> escribió: Hi again, I've added the color plot in the new cryoEF plugin release (3.0.12), which should be available in a few minutes. Best regards, Grigory -------------------------------------------------------------------------------- Grigory Sharov, Ph.D. MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK. tel. +44 (0) 1223 267228 e-mail: gs...@mr... On Fri, Jun 18, 2021 at 11:45 AM Pablo Conesa <pc...@cn...> wrote: Thanks! this is an interesting plot. Not yet available in Scipion, but I guess doable. On 18/6/21 10:10, 范宏成 wrote: Dear colleagues, I recently used the cryo-EF program in Scipion to try to quantify my protein's orientation distribution. I found it very useful compared to relion-orientation output and told me whether the preferential orientation influenced my protein's high resolution structure determination when using the single particle analysis. Recently, I found this paper <<Multifunctional graphene supports for electron cryomicroscopy>>. The Figure3 ( Mapping the surface interaction profile of the 30S ribosomal subunit on a multifunctional graphene grid) was impressive. However, I found the cryoEF output was a single color picture ( see my result in the attachment, I already had the pdb model). I am curious about how to draw this multi-color picture (especially for the surface interaction site) based on the cryo-EF results . I did't find this in the cryoEF output folder. Does anyone have some ideas or other programs in scipion which can help me find the preferential orientation site (euler angles values). Thank you! Best regards, Hongcheng Fan | | 范宏成 | | m18...@16... | 签名由 网易邮箱大师 定制 _______________________________________________ scipion-users mailing list sci...@li...https://lists.sourceforge.net/lists/listinfo/scipion-users -- Pablo Conesa - Madrid Scipion team _______________________________________________ scipion-users mailing list sci...@li... https://lists.sourceforge.net/lists/listinfo/scipion-users |
