Re: [scipion-users] How to get a multi-color orientation distribution picture from Cryo-EF

[Pablo C. <pc...@cn...>]

Thanks! this is an interesting plot. Not yet available in Scipion, but I 
guess doable.

On 18/6/21 10:10, 范宏成 wrote:
> Dear colleagues,
> I recently used the cryo-EF program in Scipion to try to quantify my 
> protein's orientation distribution. I found it very useful compared to 
> relion-orientation output and told me whether the preferential 
> orientation influenced my protein's high resolution structure 
> determination when using the single particle analysis. Recently, I 
>  found this paper <<Multifunctional graphene supports for electron 
> cryomicroscopy>>. The Figure3 ( Mapping the surface interaction 
> profile of the 30S ribosomal subunit on a multifunctional graphene 
> grid) was  impressive. However, I found the cryoEF output was a 
>  single color picture ( see my result in the attachment, I already had 
> the pdb model).  I am curious about how to draw this multi-color 
> picture (especially for  the surface interaction site)  based on the 
> cryo-EF results . I did't find this in the cryoEF output folder.  Does 
> anyone have some ideas or other programs in scipion  which can help me 
> find the preferential orientation site (euler angles values).  Thank you!
> Best regards,
> Hongcheng Fan
>
> 	
> 范宏成
> m18...@16...
>
> <https://maas.mail.163.com/dashi-web-extend/html/proSignature.html?ftlId=1&name=%E8%8C%83%E5%AE%8F%E6%88%90&uid=m18717124574%40163.com&iconUrl=https%3A%2F%2Fmail-online.nosdn.127.net%2Fsm9cc9cc9db2766bf0072c27595c17827b.jpg&items=%5B%22m18717124574%40163.com%22%5D> 
>
> 签名由 网易邮箱大师 <https://mail.163.com/dashi/dlpro.html?from=mail81> 
> 定制
>
>
> _______________________________________________
> scipion-users mailing list
> sci...@li...
> https://lists.sourceforge.net/lists/listinfo/scipion-users
-- 
Pablo Conesa - *Madrid Scipion <http://scipion.i2pc.es> team*