Re: [scipion-users] How to get a multi-color orientation distribution picture from Cryo-EF

[Grigory S. <sha...@gm...>]

Hello Hongcheng,

The color plot is currently not implemented in Scipion, thank you for
letting me know about it.

In the meantime you can use PlotOD.py script from
https://www.mrc-lmb.cam.ac.uk/crusso/cryoEF/downloads.html to generat such
a plot yourself.

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Fri, Jun 18, 2021 at 9:13 AM 范宏成 <187...@16...> wrote:

> Dear colleagues,
> I recently used the cryo-EF program in Scipion to try to quantify my
> protein's orientation distribution. I found it very useful compared to
> relion-orientation output and told me whether the preferential orientation
> influenced my protein's high resolution structure determination when using
> the single particle analysis. Recently, I  found this paper <<Multifunctional
> graphene supports for electron cryomicroscopy>>. The Figure3 ( Mapping
> the surface interaction profile of the 30S ribosomal subunit on a
> multifunctional graphene grid) was  impressive. However, I found the cryoEF
> output was a  single color picture ( see my result in the attachment, I
> already had the pdb model).  I am curious about how to draw this
> multi-color picture (especially for  the surface interaction site)  based
> on the cryo-EF results .  I did't find this in the cryoEF output folder.
> Does anyone have some ideas or other programs in scipion  which can help me
> find the preferential orientation site (euler angles values).  Thank you!
> Best regards,
> Hongcheng Fan
>
> 范宏成
> m18...@16...
>
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