[scipion-users] How to get a multi-color orientation distribution picture from Cryo-EF

[范宏成 <187...@16...>]

Dear colleagues,
I recently used the cryo-EF program in Scipion to try to quantify my protein's orientation distribution. I found it very useful compared to relion-orientation output and told me whether the preferential orientation influenced my protein's high resolution structure determination when using the single particle analysis. Recently, I  found this paper <<Multifunctional graphene supports for electron cryomicroscopy>>. The Figure3 ( Mapping the surface interaction profile of the 30S ribosomal subunit on a multifunctional graphene grid) was  impressive. However, I found the cryoEF output was a  single color picture ( see my result in the attachment, I already had the pdb model).  I am curious about how to draw this multi-color picture (especially for  the surface interaction site)  based on the cryo-EF results .  I did't find this in the cryoEF output folder.  Does anyone have some ideas or other programs in scipion  which can help me find the preferential orientation site (euler angles values).  Thank you! 
Best regards,
Hongcheng Fan


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范宏成
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m18...@16...
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