Re: [scipion-users] Extending the functionality of cryosparc2 – 3D Helical Refinement (BETA)

[Yunior C. F. R. <su...@bc...>]

Hi Dmitry,     You're right, that job can run without inputting a reference volume. The job can generate an initial volume. I have already extended that functionality but for some reason CS fails. Maybe it's my particles or the amount I'm using. I will try other sets to reach some conclusion. I'll write to you when I release the changes.     ​Best Regards,  Yunior        
                           
                            Activo
                            Vie, 30 Abr a 10:04 AM
                            ,  Mailing list for Scipion users <sci...@li...>  Escrito:
                             
Dear Yunior,
 
 
 
   
 Small
question – 
 
Is it
possible to add to the existing cryosparc2 – 3D Helical Refinement (BETA) protocol
the function to run the unsupervised helix reconstruction with our input volume
--- “when the helical refinement job also
doesn't require an input volume”?
 
 
 
   
 
As it described
here https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/helical-reconstruction-beta/case-study-empiar-10031-mavs
in the chapter Helical
Refinement — Asymmetric
Helical Refinement 
   
 Thank you 
 
 
   
 Sincerely, 
 
 
 Dmitry