Re: [scipion-users] Sidesplitter__relion postprocessing

[Grigory S. <sha...@gm...>]

Hi Dmitry,

the sidesplitter plugin was made mostly for testing purposes (since it runs
on a single iteration), so you can't use output half maps unless you run
postprocess manually outside Scipion.

The proper way to run it would be with relion plugin as described here:
https://github.com/StructuralBiology-ICLMedicine/SIDESPLITTER/blob/master/sidesplitter_wrapper.sh

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Wed, Apr 14, 2021 at 12:51 PM Dmitry Semchonok <Sem...@gm...>
wrote:

> Dear colleagues,
>
> My question is about Sidesplitter- local filter.
>
> As an output we get 2 half-maps.
>
> My question is how to use them (the half-maps) as an input in the
> relion-postprocessing protocol, since currently the previous refinement
> protocol only is required?
>
>
> Thank you!
>
>
> Sincerely,
> Dmitry
>
>
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