Re: [scipion-users] Import of star file/changes the Ang/px

[Grigory S. <sha...@gm...>]

Hello Avi,

could you attach the first 100 lines of the star files here so I can test
it?

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Tue, Apr 13, 2021 at 11:15 AM Bandyopadhyay, Abhishek <ba...@pu...>
wrote:

> Hello,
>                 I am Avi, currently working at Purdue University. I am
> using Scipion for the purpose of localized reconstruction.
>
> As I am importing my star file into Scipion using the import particles
> job, unfortunately, the program changes the Angstrom/pixel value in the
> .star file.
>
> For example, my original star file has an Ang/px value of 1.32. I then
> started a Scipion Import Particles job. I defined all the parameters (
> voltage, spehrical abberation, mag, ang/px etc), but the Scipion Import
> Particles program outputs the star file as having an Ang/px value of 1.
> Unfortunately, there was no way for me to change this at the stage of
> Define subparticles or Extract Subparticles. Both the Define Subparticles
> and Extract Subparticles jobs considered the input star file having an
> Ang/px value of 1, which is incorrect.
>
> I was wondering if you are aware of this issue and if so, could you kindly
> suggest how to tackle this issue?
>
> I managed to overcome this problem by shifting to command line operations
> for Relion reconstruct and then Relion 3DClassification. However, it would
> really be helpful if this problem can be solved at the very beginning,
> which is at the import particle step.
>
> Thank you very much for your help.
>
> Best regards,
>
> Avi
>
>
>
>
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