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From: Grigory S. <sha...@gm...> - 2021-04-13 10:18:05
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Hello Avi, could you attach the first 100 lines of the star files here so I can test it? Best regards, Grigory -------------------------------------------------------------------------------- Grigory Sharov, Ph.D. MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK. tel. +44 (0) 1223 267228 <+44%201223%20267228> e-mail: gs...@mr... On Tue, Apr 13, 2021 at 11:15 AM Bandyopadhyay, Abhishek <ba...@pu...> wrote: > Hello, > I am Avi, currently working at Purdue University. I am > using Scipion for the purpose of localized reconstruction. > > As I am importing my star file into Scipion using the import particles > job, unfortunately, the program changes the Angstrom/pixel value in the > .star file. > > For example, my original star file has an Ang/px value of 1.32. I then > started a Scipion Import Particles job. I defined all the parameters ( > voltage, spehrical abberation, mag, ang/px etc), but the Scipion Import > Particles program outputs the star file as having an Ang/px value of 1. > Unfortunately, there was no way for me to change this at the stage of > Define subparticles or Extract Subparticles. Both the Define Subparticles > and Extract Subparticles jobs considered the input star file having an > Ang/px value of 1, which is incorrect. > > I was wondering if you are aware of this issue and if so, could you kindly > suggest how to tackle this issue? > > I managed to overcome this problem by shifting to command line operations > for Relion reconstruct and then Relion 3DClassification. However, it would > really be helpful if this problem can be solved at the very beginning, > which is at the import particle step. > > Thank you very much for your help. > > Best regards, > > Avi > > > > > _______________________________________________ > scipion-users mailing list > sci...@li... > https://lists.sourceforge.net/lists/listinfo/scipion-users > |