Re: [scipion-users] Observation for relion-motion correction and MotionCorr - movie alignment - correct doze "per Frame"

[Pablo C. <pc...@cn...>]

Thanks Dmitry. Good to know.

On 12/3/21 11:32, Dmitry Semchonok wrote:
> Dear colleagues,
>
> Small Friday observation.
>
> I just found the reason why the output frames from either "relion-motion correction" or “motioncorr - movie alignment" protocols can be totally black.
>
> The reason was the the doze "per Frame" in the previous step - "import movies” that was not set correctly (by the user): so, instead of about 1 e/A^2 (depending on the recording settings) or, in my case 0.9856 due to type was set 9856.0
>
> Interestingly, even with those wrong settings only xmipp - Flex align (on the same movies dataset) gave the nice output images (not the black ones). :)
>
>
>
>
> Sincerely,
> Dmitry
>
>
>
>
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-- 
Pablo Conesa - *Madrid Scipion <http://scipion.i2pc.es> team*