[scipion-users] Observation for relion-motion correction and MotionCorr - movie alignment - correct doze "per Frame"

[Dmitry S. <Sem...@gm...>]

Dear colleagues,

Small Friday observation. 

I just found the reason why the output frames from either "relion-motion correction" or “motioncorr - movie alignment" protocols can be totally black.

The reason was the the doze "per Frame" in the previous step - "import movies” that was not set correctly (by the user): so, instead of about 1 e/A^2 (depending on the recording settings) or, in my case 0.9856 due to type was set 9856.0

Interestingly, even with those wrong settings only xmipp - Flex align (on the same movies dataset) gave the nice output images (not the black ones). :)




Sincerely,
Dmitry