Re: [scipion-users] (no subject)

[Yunior C. F. R. <cfo...@cn...>]

Hi Dmitry,

I followed the steps in your email and everything worked for me. I 
attach an image. For the symmetry expansion algorithm, tomorrow you will 
have a protocol inside the plugin, hehehehehe.
On the other hand, what version of relion do you have installed?
I've been reported problems with particles coming from relion3.1, but 
some CS algorithms require these particles to have CTF and alignment.

Do you want us to organise a meeting to see what mistakes you have?

Best Regards,

Yunior

El 8/3/21 a las 21:39, Dmitry A. Semchonok escribió:
>
> Dear Yunior and all,
>
> I try to run the local refinement by cryosparc plugin in SCIPION.
>
> Before that I need to run the symmetry expansion.
>
> But the cryosparc symmetry expansion plugin is currently not available 
> in SCIPION.
>
> So what I tried:
>
>  1. Run the symmetry expansion in relion. Then the local refinement in
>     cryosparc.
>
> But I got the error about the absence of CTF information.
>
>  2. Run the symmetry expansion in cryosparc as a continuation of for
>     example  3D -refinement (with debug function on).
>
> The symmetry expansion works fine. But then the locar refinement in 
> cryosparc gives an error:
>
> /Traceback (most recent call last):/
>
> /  File "cryosparc_worker/cryosparc_compute/run.py", line 84, in 
> cryosparc_compute.run.main/
>
> /  File 
> "cryosparc_worker/cryosparc_compute/jobs/local_refine/newrun.py", line 
> 116, in cryosparc_compute.jobs.local_refine.newrun.run_local_refine/
>
> /  File 
> "/home/panos/software/cryosparc/cryosparc2_worker/cryosparc_compute/particles.py", 
> line 77, in read_blobs/
>
> /    with open(abs_path, 'rb') as mrc_file:/
>
> /FileNotFoundError: [Errno 2] No such file or directory: 
> '/home/panos/software/cryosparc/scipion_projects/Fraction_33_Lisa-panos/P18/J129/imported/mic_000001.mrcs'/
>
> Is there any possibility to run the local refinement after the 
> symmetry expansion?
>
> Thank you.
>
> Sincerely,
>
> Dmitry
>
> Sent from Mail <https://go.microsoft.com/fwlink/?LinkId=550986> for 
> Windows 10
>