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[scipion-users] sphire – cryolo import & sphire - cryolo picking
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From: Dmitry A. S. <sem...@gm...> - 2021-03-06 07:50:32
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<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=utf-8"><meta name=Generator content="Microsoft Word 15 (filtered medium)"><style><!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {margin:0in; font-size:11.0pt; font-family:"Calibri",sans-serif;} a:link, span.MsoHyperlink {mso-style-priority:99; color:blue; text-decoration:underline;} .MsoChpDefault {mso-style-type:export-only;} @page WordSection1 {size:8.5in 11.0in; margin:1.0in 1.0in 1.0in 1.0in;} div.WordSection1 {page:WordSection1;} --></style></head><body lang=EN-US link=blue vlink="#954F72" style='word-wrap:break-word'><div class=WordSection1><div><p class=MsoNormal>Dear colleagues,</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>How does the import sphire – cryolo import works?</p><p class=MsoNormal>Using this protocol, I imported the volume that I want to use for picking with shire picking.</p><p class=MsoNormal>But the shire picking gives an error.</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><span style='color:red'>Protocol failed: Command 'eval "$(/opt/Scipion3/software/em/eman-2.31/condabin/conda shell.bash hook)"&& conda activate cryolo-1.7.6 && cryolo_predict.py -c Runs/003144_SphireProtCRYOLOPicking/extra/config.json -w /data1/ScipionUserData/projects/Fraction_33_Lisa/Runs/003113_SphireProtCryoloImport/extra/cryosparc_P18_J64_005_volume_map.mrc -i Runs/003144_SphireProtCRYOLOPicking/tmp/micrographs_1-17/ -o Runs/003144_SphireProtCRYOLOPicking/tmp/micrographs_1-17/ -t 0.200 -g 0 -nc 4' returned non-zero exit status 1.<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Could you give any suggestions?</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Thank you</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Sincerely,</p><p class=MsoNormal>Dmitry </p></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Sent from <a href="https://go.microsoft.com/fwlink/?LinkId=550986">Mail</a> for Windows 10</p><p class=MsoNormal><o:p> </o:p></p></div></body></html> |
