Re: [scipion-users] Bayesian polishing issue

[Grigory S. <sha...@gm...>]

CC for the records:
The problem was in xmipp center particles protocol that did not preserve
micnames. I've reported this to the xmipp dev team which will be on it.


Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gs...@mr...


On Wed, Dec 30, 2020 at 4:23 PM Dmitry A. Semchonok <sem...@gm...>
wrote:

> Thank you very much, Grigory, for your detailed investigation and
> recommendations!
>
>
> Sincerely,
> Dmitry
>
> On December 30, 2020 6:16:12 PM Grigory Sharov <sha...@gm...>
> wrote:
>
>> Hi, just confirmed, polishing fails with the same error as you reported.
>> I'll inform the xmipp team to fix this. Thanks a lot for reporting this!
>>
>> In Relion motioncor you set the number of patches to 1x1 which means you
>> only did global movie alignment. It's okay, but probably better results can
>> be obtained with a number of patches 5x5 (see relion tutorial).
>>
>> Best regards,
>> Grigory
>>
>>
>> --------------------------------------------------------------------------------
>> Grigory Sharov, Ph.D.
>>
>> MRC Laboratory of Molecular Biology,
>> Francis Crick Avenue,
>> Cambridge Biomedical Campus,
>> Cambridge CB2 0QH, UK.
>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>> e-mail: gs...@mr...
>>
>>
>> On Wed, Dec 30, 2020 at 4:11 PM Dmitry A. Semchonok <sem...@gm...>
>> wrote:
>>
>>> Dear Grigory,
>>>
>>> Thank you!
>>>
>>> So now it is clear that the xmipp centering doesnot preserve the
>>> required info for Bayesian polishing - micName.
>>> Maybe it can be corrected by Scipion/xmipp team sometime?
>>>
>>> I have a question - what do you mean by
>>>
>>> 1) you run only global mc without local patch-based alignment
>>>>
>>>
>>> Usually I run relion - motioncor protocol for the motion alignment, and
>>> continue further with the image processing.
>>>
>>> Am I doing something wrong?
>>>
>>> What could you recommend to run inorder to correct for the motion?
>>>
>>> Thank you!
>>>
>>> Sincerely,
>>> Dmitry
>>>
>>> On December 30, 2020 5:47:22 PM Grigory Sharov <sha...@gm...>
>>> wrote:
>>>
>>>> Hi again,
>>>>
>>>> I think I found the culprit which is xmipp center particles protocol.
>>>> It does not preserve coordinate micName. I will run polishing to confirm.
>>>>
>>>> PS. Some things I've noticed:
>>>> 1) you run only global mc without local patch-based alignment
>>>> 2) for all cryosparc protocols numbers of mpi/threads are irrelevant
>>>> since they are not available in cryosparc for modification. So you could
>>>> use always 1 mpi, 1 thread
>>>>
>>>>
>>>> Best regards,
>>>> Grigory
>>>>
>>>>
>>>> --------------------------------------------------------------------------------
>>>> Grigory Sharov, Ph.D.
>>>>
>>>> MRC Laboratory of Molecular Biology,
>>>> Francis Crick Avenue,
>>>> Cambridge Biomedical Campus,
>>>> Cambridge CB2 0QH, UK.
>>>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>>>> e-mail: gs...@mr...
>>>>
>>>>
>>>> On Wed, Dec 30, 2020 at 2:20 PM Grigory Sharov <
>>>> sha...@gm...> wrote:
>>>>
>>>>> Thanks! That's quite a workflow. I did a simple test (below) already
>>>>> and it worked. So must be some other combination of protocols. I'll try to
>>>>> follow your project with my test data.
>>>>> [image: image.png]
>>>>> Best regards,
>>>>> Grigory
>>>>>
>>>>>
>>>>> --------------------------------------------------------------------------------
>>>>> Grigory Sharov, Ph.D.
>>>>>
>>>>> MRC Laboratory of Molecular Biology,
>>>>> Francis Crick Avenue,
>>>>> Cambridge Biomedical Campus,
>>>>> Cambridge CB2 0QH, UK.
>>>>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>>>>> e-mail: gs...@mr...
>>>>>
>>>>>
>>>>> On Wed, Dec 30, 2020 at 1:59 PM Dmitry Semchonok <sem...@gm...>
>>>>> wrote:
>>>>>
>>>>>> Dear Grigory,
>>>>>>
>>>>>>
>>>>>>
>>>>>> Please find the file in the attachment.
>>>>>>
>>>>>>
>>>>>>
>>>>>> I also progressed further, trying to re-extract the good particles
>>>>>> and run the Bayesian polishing in relion protocols above only (not yet
>>>>>> there).
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sincerely,
>>>>>>
>>>>>> Dmitry
>>>>>>
>>>>>>
>>>>>>
>>>>>> *От: *Grigory Sharov <sha...@gm...>
>>>>>> *Отправлено: *30 декабря 2020 г. в 14:51
>>>>>> *Кому: *Dmitry A. Semchonok <sem...@gm...>
>>>>>> *Тема: *Re: [scipion-users] Bayesian polishing issue
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sure, you click Ctrl+A to select all protocols, then press Export and
>>>>>> choose a json file to save. The file will be very small so you can attach
>>>>>> it to the email.
>>>>>>
>>>>>> Thanks!
>>>>>>
>>>>>>
>>>>>>
>>>>>> Best regards,
>>>>>> Grigory
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> --------------------------------------------------------------------------------
>>>>>>
>>>>>> Grigory Sharov, Ph.D.
>>>>>>
>>>>>> MRC Laboratory of Molecular Biology,
>>>>>> Francis Crick Avenue,
>>>>>> Cambridge Biomedical Campus,
>>>>>> Cambridge CB2 0QH, UK.
>>>>>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>>>>>>
>>>>>> e-mail: gs...@mr...
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Wed, Dec 30, 2020 at 12:50 PM Dmitry A. Semchonok <
>>>>>> sem...@gm...> wrote:
>>>>>>
>>>>>> Dear Grigory,
>>>>>>
>>>>>>
>>>>>>
>>>>>> I already thought about that, but I forgot how to do that.
>>>>>>
>>>>>>
>>>>>>
>>>>>> Could you please remind me how can I copy my workflow?
>>>>>>
>>>>>>
>>>>>>
>>>>>> Thank you,
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sincerely,
>>>>>>
>>>>>> Dmitry
>>>>>>
>>>>>>
>>>>>>
>>>>>> On December 30, 2020 2:10:53 PM Grigory Sharov <
>>>>>> sha...@gm...> wrote:
>>>>>>
>>>>>> Hi Dmitry,
>>>>>>
>>>>>>
>>>>>>
>>>>>> Could you possibly send me your workflow for the project? Since there
>>>>>> are so many cryosparc protocols and I'm not familiar with the code it will
>>>>>> take time to figure it out.
>>>>>>
>>>>>>
>>>>>>
>>>>>> Grigory
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Tue, Dec 29, 2020, 14:54 Dmitry Semchonok <sem...@gm...>
>>>>>> wrote:
>>>>>>
>>>>>> I run the motion correction with relion – motioncor
>>>>>>
>>>>>> Is there a way to do the cryosparc-  motion correction inside the
>>>>>> SCIPON3 (I was not aware) ?
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sincerely,
>>>>>>
>>>>>> Dmitry
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> *От: *Grigory Sharov <sha...@gm...>
>>>>>> *Отправлено: *29 декабря 2020 г. в 16:45
>>>>>> *Кому: *Mailing list for Scipion users
>>>>>> <sci...@li...>
>>>>>> *Тема: *Re: [scipion-users] Bayesian polishing issue
>>>>>>
>>>>>>
>>>>>>
>>>>>> Oh, I see. You are probably one of the first users trying to combine
>>>>>> cryosparc and relion protocols in scipion. I will investigate whether
>>>>>> cryosparc plugin does not keep the original micrographName and get back to
>>>>>> you.
>>>>>>
>>>>>>
>>>>>>
>>>>>> Did you run motion correction also in cryosparc or in relion?
>>>>>>
>>>>>>
>>>>>> Best regards,
>>>>>> Grigory
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> --------------------------------------------------------------------------------
>>>>>>
>>>>>> Grigory Sharov, Ph.D.
>>>>>>
>>>>>> MRC Laboratory of Molecular Biology,
>>>>>> Francis Crick Avenue,
>>>>>> Cambridge Biomedical Campus,
>>>>>> Cambridge CB2 0QH, UK.
>>>>>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>>>>>>
>>>>>> e-mail: gs...@mr...
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Tue, Dec 29, 2020 at 2:36 PM Dmitry Semchonok <sem...@gm...>
>>>>>> wrote:
>>>>>>
>>>>>> Dear Grigory,
>>>>>>
>>>>>>
>>>>>>
>>>>>> Thank you.
>>>>>>
>>>>>>
>>>>>>
>>>>>> That is quite interesting result.
>>>>>>
>>>>>> Could you please check the new Bayesian run (I  copied the files and
>>>>>> will send you with Wetransfer again)
>>>>>>
>>>>>>
>>>>>>
>>>>>> If the problem remains the same it means that the cryosparc 3D refine
>>>>>> output can not be followed up with relion Bayesian polishing.
>>>>>>
>>>>>>
>>>>>>
>>>>>> Please see the project tree.
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> What is your opinion?
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sincerely,
>>>>>>
>>>>>> Dmitry
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> Отправлено из приложения "Почта
>>>>>> <https://go.microsoft.com/fwlink/?LinkId=550986>" для Windows 10
>>>>>>
>>>>>>
>>>>>>
>>>>>> *От: *Grigory Sharov <sha...@gm...>
>>>>>> *Отправлено: *29 декабря 2020 г. в 16:22
>>>>>> *Кому: *Mailing list for Scipion users
>>>>>> <sci...@li...>
>>>>>> *Тема: *Re: [scipion-users] Bayesian polishing issue
>>>>>>
>>>>>>
>>>>>>
>>>>>> Hi,
>>>>>>
>>>>>>
>>>>>>
>>>>>> Thank you for sending over the files. rlnMicrographName in the
>>>>>> input_particles.star and input_corrected_micrographs.star are completely
>>>>>> different. I do not know how you have generated your particles, but you
>>>>>> cannot run polishing with them.
>>>>>>
>>>>>>
>>>>>>
>>>>>> rlnMicrographName
>>>>>>
>>>>>> Best regards,
>>>>>> Grigory
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> --------------------------------------------------------------------------------
>>>>>>
>>>>>> Grigory Sharov, Ph.D.
>>>>>>
>>>>>> MRC Laboratory of Molecular Biology,
>>>>>> Francis Crick Avenue,
>>>>>> Cambridge Biomedical Campus,
>>>>>> Cambridge CB2 0QH, UK.
>>>>>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>>>>>>
>>>>>> e-mail: gs...@mr...
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Tue, Dec 29, 2020 at 11:32 AM Grigory Sharov <
>>>>>> sha...@gm...> wrote:
>>>>>>
>>>>>> Hi,
>>>>>>
>>>>>>
>>>>>>
>>>>>> You forgot to attach Runs/004138_ProtRelionBayesianPolishing/input_particles.star
>>>>>> file
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Tue, Dec 29, 2020, 11:26 Dmitry Semchonok <sem...@gm...>
>>>>>> wrote:
>>>>>>
>>>>>> Dear Grigory,
>>>>>>
>>>>>>
>>>>>>
>>>>>> Please find the file attached
>>>>>>
>>>>>>
>>>>>>
>>>>>> Thank you!
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sincerely,
>>>>>>
>>>>>> Dmitry
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Tue, Dec 29, 2020 at 12:28 AM Grigory Sharov <
>>>>>> sha...@gm...> wrote:
>>>>>>
>>>>>> Hi Dmitry,
>>>>>>
>>>>>>
>>>>>>
>>>>>> this usually happens if you have moved your raw movie files from
>>>>>> where Scipion expects them to be.
>>>>>>
>>>>>>
>>>>>>
>>>>>> Could you please send me
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_particles.star and
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_corrected_micrographs.star
>>>>>> files?
>>>>>>
>>>>>>
>>>>>> Best regards,
>>>>>> Grigory
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> --------------------------------------------------------------------------------
>>>>>>
>>>>>> Grigory Sharov, Ph.D.
>>>>>>
>>>>>> MRC Laboratory of Molecular Biology,
>>>>>> Francis Crick Avenue,
>>>>>> Cambridge Biomedical Campus,
>>>>>> Cambridge CB2 0QH, UK.
>>>>>> tel. +44 (0) 1223 267228 <+44%201223%20267228>
>>>>>>
>>>>>> e-mail: gs...@mr...
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Mon, Dec 28, 2020 at 10:16 PM Dmitry Semchonok <
>>>>>> sem...@gm...> wrote:
>>>>>>
>>>>>> Dear colleagues,
>>>>>>
>>>>>>
>>>>>>
>>>>>> *Bayesian polishing issue –*
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> 00008:   workingDir: Runs/004138_ProtRelionBayesianPolishing
>>>>>>
>>>>>> 00009:   runMode: Restart
>>>>>>
>>>>>> 00010:             MPI: 1
>>>>>>
>>>>>> 00011:         threads: 14
>>>>>>
>>>>>> 00012:    Starting at step: 1
>>>>>>
>>>>>> 00013:    Running steps
>>>>>>
>>>>>> 00014:   STARTED: convertInputStep, step 1, time 2020-12-28
>>>>>> 23:03:24.304754
>>>>>>
>>>>>> 00015:   Converting set from
>>>>>> 'Runs/003937_ProtRelionRefine3D/particles.sqlite' into
>>>>>> 'Runs/004138_ProtRelionBayesianPolishing/input_particles.star'
>>>>>>
>>>>>> 00016:   convertBinaryFiles: creating soft links.
>>>>>>
>>>>>> 00017:      Root:
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input/particles/input ->
>>>>>> Runs/000427_ProtRelionExtractParticles/extra
>>>>>>
>>>>>> 00018:   FINISHED: convertInputStep, step 1, time 2020-12-28
>>>>>> 23:04:33.416996
>>>>>>
>>>>>> 00019:   STARTED: trainOrPolishStep, step 2, time 2020-12-28
>>>>>> 23:04:33.422574
>>>>>>
>>>>>> 00020:   relion_motion_refine --i
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_particles.star --o
>>>>>> Runs/004138_ProtRelionBayesianPolishing/extra --f
>>>>>> Runs/004089_ProtRelionPostprocess/extra/postprocess.star --angpix_ref
>>>>>> 0.59200 --corr_mic
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_corrected_micrographs.star
>>>>>> --first_frame 3 --last_frame 0 --min_p 421029 --eval_frac 0.500
>>>>>> --align_frac 0.500 --params3 --j 14
>>>>>>
>>>>>> 00021:    + Reading
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_particles.star..
>>>>>>
>>>>>> 00022:   in:
>>>>>> /home/user/Data/Software/Scipion3/software/em/relion-3.1.0/src/jaz/micrograph_handler.cpp,
>>>>>> line 261
>>>>>>
>>>>>> 00023:   ERROR:
>>>>>>
>>>>>> 00024:   There is no movie metadata STAR file for any micrographs!
>>>>>>
>>>>>> 00025:   === Backtrace  ===
>>>>>>
>>>>>> 00026:   relion_motion_refine(_ZN11RelionErrorC1ERKSsS1_l+0x41)
>>>>>> [0x453571]
>>>>>>
>>>>>> 00027:
>>>>>> relion_motion_refine(_ZN17MicrographHandler11loadInitialERKSt6vectorI13MetaDataTableSaIS1_EEbRiRdRSs+0xb95)
>>>>>> [0x531275]
>>>>>>
>>>>>> 00028:
>>>>>> relion_motion_refine(_ZN17MicrographHandler4initERKSt6vectorI13MetaDataTableSaIS1_EEbiRiRdRSs+0x78)
>>>>>> [0x533a88]
>>>>>>
>>>>>> 00029:   relion_motion_refine(_ZN13MotionRefiner4initEv+0x546)
>>>>>> [0x47d1a6]
>>>>>>
>>>>>> 00030:   relion_motion_refine(main+0x31) [0x428841]
>>>>>>
>>>>>> 00031:   /lib64/libc.so.6(__libc_start_main+0xf5) [0x7ff26cfb7555]
>>>>>>
>>>>>> 00032:   relion_motion_refine() [0x42955f]
>>>>>>
>>>>>> 00033:   ==================
>>>>>>
>>>>>> 00034:   ERROR:
>>>>>>
>>>>>> 00035:   There is no movie metadata STAR file for any micrographs!
>>>>>>
>>>>>> 00036:   Traceback (most recent call last):
>>>>>>
>>>>>> 00037:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/protocol/protocol.py",
>>>>>> line 189, in run
>>>>>>
>>>>>> 00038:       self._run()
>>>>>>
>>>>>> 00039:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/protocol/protocol.py",
>>>>>> line 240, in _run
>>>>>>
>>>>>> 00040:       resultFiles = self._runFunc()
>>>>>>
>>>>>> 00041:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/protocol/protocol.py",
>>>>>> line 236, in _runFunc
>>>>>>
>>>>>> 00042:       return self._func(*self._args)
>>>>>>
>>>>>> 00043:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/relion/protocols/protocol_bayesian_polishing.py",
>>>>>> line 309, in trainOrPolishStep
>>>>>>
>>>>>> 00044:       self.runJob(prog, args)
>>>>>>
>>>>>> 00045:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/protocol/protocol.py",
>>>>>> line 1357, in runJob
>>>>>>
>>>>>> 00046:       self._stepsExecutor.runJob(self._log, program,
>>>>>> arguments, **kwargs)
>>>>>>
>>>>>> 00047:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/protocol/executor.py",
>>>>>> line 65, in runJob
>>>>>>
>>>>>> 00048:       process.runJob(log, programName, params,
>>>>>>
>>>>>> 00049:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/utils/process.py",
>>>>>> line 51, in runJob
>>>>>>
>>>>>> 00050:       return runCommand(command, env, cwd)
>>>>>>
>>>>>> 00051:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/site-packages/pyworkflow/utils/process.py",
>>>>>> line 66, in runCommand
>>>>>>
>>>>>> 00052:       check_call(command, shell=True, stdout=sys.stdout,
>>>>>> stderr=sys.stderr,
>>>>>>
>>>>>> 00053:     File
>>>>>> "/home/user/.conda/envs/.scipion3env/lib/python3.8/subprocess.py", line
>>>>>> 364, in check_call
>>>>>>
>>>>>> 00054:       raise CalledProcessError(retcode, cmd)
>>>>>>
>>>>>> 00055:   subprocess.CalledProcessError: Command 'relion_motion_refine
>>>>>> --i Runs/004138_ProtRelionBayesianPolishing/input_particles.star --o
>>>>>> Runs/004138_ProtRelionBayesianPolishing/extra --f
>>>>>> Runs/004089_ProtRelionPostprocess/extra/postprocess.star --angpix_ref
>>>>>> 0.59200 --corr_mic
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_corrected_micrographs.star
>>>>>> --first_frame 3 --last_frame 0 --min_p 421029 --eval_frac 0.500
>>>>>> --align_frac 0.500 --params3 --j 14 ' returned non-zero exit status 1.
>>>>>>
>>>>>> 00056:   Protocol failed: Command 'relion_motion_refine --i
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_particles.star --o
>>>>>> Runs/004138_ProtRelionBayesianPolishing/extra --f
>>>>>> Runs/004089_ProtRelionPostprocess/extra/postprocess.star --angpix_ref
>>>>>> 0.59200 --corr_mic
>>>>>> Runs/004138_ProtRelionBayesianPolishing/input_corrected_micrographs.star
>>>>>> --first_frame 3 --last_frame 0 --min_p 421029 --eval_frac 0.500
>>>>>> --align_frac 0.500 --params3 --j 14 ' returned non-zero exit status 1.
>>>>>>
>>>>>> 00057:   FAILED: trainOrPolishStep, step 2, time 2020-12-28
>>>>>> 23:04:45.953315
>>>>>>
>>>>>> 00058:   *** Last status is failed
>>>>>>
>>>>>> 00059:   ------------------- PROTOCOL FAILED (DONE 2/2)
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> Any ideas?
>>>>>>
>>>>>> Thank you!
>>>>>>
>>>>>>
>>>>>>
>>>>>> Sincerely,
>>>>>>
>>>>>> Dmitry
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> _______________________________________________
>>>>>> scipion-users mailing list
>>>>>> sci...@li...
>>>>>> https://lists.sourceforge.net/lists/listinfo/scipion-users
>>>>>>
>>>>>> _______________________________________________
>>>>>> scipion-users mailing list
>>>>>> sci...@li...
>>>>>> https://lists.sourceforge.net/lists/listinfo/scipion-users
>>>>>>
>>>>>> _______________________________________________
>>>>>> scipion-users mailing list
>>>>>> sci...@li...
>>>>>> https://lists.sourceforge.net/lists/listinfo/scipion-users
>>>>>>
>>>>>>
>>>>>>
>>>>>> _______________________________________________
>>>>>> scipion-users mailing list
>>>>>> sci...@li...
>>>>>> https://lists.sourceforge.net/lists/listinfo/scipion-users
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>
>>>
>
>
>