Re: [scipion-users] Localized reconstruction Scipion 3.0

[Grigory S. <sha...@gm...>]

Hello Nicholas,

It took me a while to figure it out, but the problem is now fixed in the
latest version of relion plugin.

Grigory

On Wed, Oct 7, 2020, 02:24 Nicholas Prokhorov <pro...@gm...> wrote:

> Dear all,
>
> I’m trying to use Localized Reconstruction plugin in Scipion 3. I imported
> particles from Relion 3.1, defined subparticles, filtered them and
> extracted with a new box size. Everything worked seemingly okay. But when I
> try and do 2D or 3D classification or 3D auto-refine the subparticles are
> treated as if they have the size of the original imported particles - 972
> px instead of 168 px. For instance, in 3D auto-refine I have "ERROR The
> reference box size is 168 A, but the box size of the first optics group of
> the data is 972 A!” (which is btw not correct as the reference box size is
> 168 px and the pixel size is 1.1 A) Particles in particles.mrcs have
> correct dimensions and the problem seems to be in metadata. I opened
> particles.sqlite from the extraction job and it looks like every particle
> has its own relion optics group header. I changed rlnImageSize from 972 to
> 168 in all entries but still have the same error from 3D auto-refine when I
> try using corrected particles.sqlite. What would be the simplest way to
> overcome the bug?
>
> Thank you,
>
> Nikolai
>
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