Re: [scipion-users] default density threshold of 2D class averages in SCIPION

[Grigory S. <sha...@gm...>]

Hi,

There's no such thing as a density threshold for 2D classification in
relion, nor any projection operations.

On Fri, Nov 6, 2020, 20:44 Dmitry A. Semchonok <sem...@gm...> wrote:

> Dear colleagues,
>
>
> I am kindly asking for your advice.
>
>
> *What are the default parameters for visualizing the 2D class averages in
> SCIPION and what is the default density threshold?*
>
>
>
> This question is connected to the possibility to see (or not) the
> structures we are interested in on the 2D class averages, projected in
> SCIPION.
>
> There is a doubt that if the density threshold differs for different 2D
> class averages in the series of the 2D alignments, some parts of the
> structure may not be visible and/or comparable.
>
>
>
> The 2D classifications were done using relion 2D classification protocol
> inside the SCIPION.
>
>
> Thank you in advance.
>
>
>
> Sincerely,
>
> Dmitry
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