From: Dmitry A. S. <sem...@gm...> - 2020-11-06 20:44:36
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Dear colleagues, I am kindly asking for your advice. What are the default parameters for visualizing the 2D class averages in SCIPION and what is the default density threshold? This question is connected to the possibility to see (or not) the structures we are interested in on the 2D class averages, projected in SCIPION. There is a doubt that if the density threshold differs for different 2D class averages in the series of the 2D alignments, some parts of the structure may not be visible and/or comparable. The 2D classifications were done using relion 2D classification protocol inside the SCIPION. Thank you in advance. Sincerely, Dmitry |