[scipion-users] Localized reconstruction Scipion 3.0

[Nicholas P. <pro...@gm...>]

Dear all,

I’m trying to use Localized Reconstruction plugin in Scipion 3. I imported particles from Relion 3.1, defined subparticles, filtered them and extracted with a new box size. Everything worked seemingly okay. But when I try and do 2D or 3D classification or 3D auto-refine the subparticles are treated as if they have the size of the original imported particles - 972 px instead of 168 px. For instance, in 3D auto-refine I have "ERROR The reference box size is 168 A, but the box size of the first optics group of the data is 972 A!” (which is btw not correct as the reference box size is 168 px and the pixel size is 1.1 A) Particles in particles.mrcs have correct dimensions and the problem seems to be in metadata. I opened particles.sqlite from the extraction job and it looks like every particle has its own relion optics group header. I changed rlnImageSize from 972 to 168 in all entries but still have the same error from 3D auto-refine when I try using corrected particles.sqlite. What would be the simplest way to overcome the bug?

Thank you,

Nikolai